Promatranje of bakterija treba biti done korištenje an optički mikroskop
As a precision instrument for povećalo i promatranje objekti, optički mikroskopi can observe different types of samples. Users in industries such as microbial research, plant research, and animal research need to see the level of size. Therefore, when observing such samples, what kind of microscope should be used? This article provides a detailed analysis.
The answer to this question can be discussed from aspects such as the size of the bacterial body, the resolution of the human eye and microscope.
1. The size of bacterial bodies is generally in the micrometer scale, taking Escherichia coli as an example: the length is about 1um and the width is about 0.5um.
2. Rezolucija Odnosi to the small distance between two object points that can be clearly distinguished.
The resolution of a microscope resor to the small distance between two object points that can be clear distinguished after being uvećano i imaged by the microscope.
The resolution of an instrument referers to its ability to provide microstructure information of the measured object. The higher the resolution, the more detailed the information provided. The amplification beyond the amplification limit is called invalid amplification, which cannot provide more detailed information about the structure.
3. The resolution of The Human Eye Is 0.1mm, which means that under sufficient light and a distance of 1 foot, the minimum distance that the Human Eye Can Distinguish Between Two Points Is 0.1mm.
4. In sažetak, to see the Presence of Bacteria, The Magnification must be at least 0.1mm ÷ 0.5um=1000} ÷ 5=200 times. Međutim, at this magnification, the bacteria (Escherichia coli) seen are a small dot, and in order to see more detailed struktural information, it is necessary to increase the the magnification of the microscope.
5. The resolution of an obični optički mikroskop is limited by the wavelength of visible light (390-770nm) and generally does not exceed 1000 times, which is the amplification limit of an ordinary optical microscope. Magnification exceeding 1000 times cannot provide more detailed struktural information, which is within the scope of ineffective amplification.
6. The uvećanje of an elektron mikroskop can reach 800000 times, i its Basic Principle Is The Same As That of An Ordinary Optical Microscope, Which Generrates Short WaveLength Electron WaveLength Electron Waves Through High Pressure.
By zooming in at 400 times (10x, 40x), you can barely see bacteria, but they are only the size of a needle tip, like small dots. Općenito, we still zoom in to 1000 times (10x, 100x) for observation, at this time, the appearance of the bacteria can still be clearly seen, even after special staining of flagella. Uvećanje 1000 times requires the use of an ulje leća. The takozvano ulje leća refers to dropping a drop of cedar ulje between the objective lens and the cover glass. The light refractive index of cedar oil is higher than that of air, so that a larger magnification can be used.
10x and 40x indicate that the the lens is MAGNIFIED BY 10x And 40x. The magnification of the eyepiece is Umnože BY The Uveća of The Objective Lens to OBT The Total MAGNIFICATION of This Microscope. The eyepiece and Objective leća of A High School Microscope can be replaced, and the eyepiece is Usually 5x or 10x. The eyepiece of a small microscope is obično only 10x, and it has been examined for E. coli, Staphylococcus aureus, Bacillus subtilis, and Bacillus megaterium (used to observe spores).
